Fig. 5

Acid-PAGE of T1 grains showing altered gliadin protein profiles. Gliadin extracts from Fielder-CRISPR T1 grain from each of the 4 constructs were run on non-denaturing Acid-PAGE alongside the gliadin extract from Fielder wild type. Each panel represent a different gel. The lanes displayed next to each other have been run alongside each other. The start of the sample names refers to the constructs with the sgRNAs they include (α1, α2, γ3 or α2γ3), followed by the T0 plant line and grain number. The black and grey arrows point respectively at the changes intended or unintended by the construct present in the plant that set the grains. a α-gliadin bands missing likely related to mutations on Chr6-AS, b α-gliadin bands missing or lower expressed likely related to mutations on Chr6 in all 3 homoeologous genomes, c ω-gliadin bands shifted up and γ-gliadin band with lower expression level likely related to mutations on Chr1-BS or DS, d ω- γ- and α-gliadin bands with lower expression likely related to mutations on Chr6-AS, e) ω-gliadin bands shifted up and γ-gliadin bands missing likely to be related to Chr1-AS, similar mutant profile in two different T1 grains from the same T0 plant.