Acid-PAGE of Chinese Spring deletion lines showing altered endosperm gliadin protein profiles. Gliadin extracts from grains of Chinese Spring nullisomic/tetrasomic lines and Kansas deletion lines were run on non-denaturing Acid-PAGE alongside a gliadin extract from Chinese Spring (CS WT). The lanes displayed next to each other have been run alongside each other on the same gel but each panel represents a different gel. Each sample was always run alongside CS WT as a control. The black and grey arrows point at the changes in the protein groups from the deleted chromosome arms and in others respectively. CS gliadin profile in absence of a Chr1-AS, b Chr1-BS, c Chr1-DS, d part of Chr6-AS, e Chr6-DS, f part of Chr1-BS and Chr6-DS.