Transgenerational memory of gene expression changes induced by heavy metal stress in rice (Oryza sativa L.)

BMC Plant Biology

Table 1 Gene expression changes observed for the 18 functionally diverse random genes in heavy metal treated seedlings of rice cv. Matsumae (S₀ generation)

Gene name	Genbank acc.^a	Chr.^a	Gene expression changes observed in heavy metal treated plants of S₀ generation^b
			Cu²⁺(1000 μm . L⁻¹)	Cd²⁺ (1000 μm . L⁻¹)	Cr³⁺ (1000 μm . L^− 1)	Hg²⁺ (50 μm . L^− 1)	Freq. (%)^c
Transposable elements (TEs)
Tos17	AC087545^(Tos17A)	10	U	U	U	U	100
Tos17	AP008213^(Tos17B)	7	U	U	U	U	100
Osr42	AF458768	4	U	N	U	U	75
Low copy protein-coding genes
Homeobox gene	AB007627	2	U	U	U	U	100
DNA-binding protein	X88798	5	U	U	U	U	100
Elongation factor	D12821	7	U	U	U	U	100
HSP70	X67711	11	U	U	U	U	100
SNF-FZ14	DQ239432	7	U	U	U	U	100
S3	AY328087	12	N	N	N	N	0
YF25	DQ239435	11	D	D	D	D	100
Rice P_1B subfamily of Heavy Metal-transporting P-type ATPases (HMAs)
OsHMA1	AP003935	6	N	U	N	U	50
OsHMA2	AP004278	6	U	N	N	N	25
OsHMA3	AP005246	7	–	–	–	–	–
OsHMA4	AP004184	2	N	N	N	N	0
OsHMA5	AL606647	4	U	U	N	U	75
OsHMA6	AP004836	2	U	U	U	U	100
OsHMA7	AP004376	8	U	U	U	U	100
OsHMA8	AC125472	3	N	N	U	U	50
OsHMA9	AP008212	6	N	U	N	U	50
	Total^d (%)		72.2	72.2	66.7	83.3

Note: ^aDetermined by BlastN searches performed at NCBI
^bChanges in gene expression pattern were defined as: N = No change in gene expression; U = Significantly up-regulated expression; D = Significant down-regulated expression; and - = No expression
^CNumber of times a gene responded similarly to different heavy metal stresses; represented as percentage in the table
^d Number of times a heavy metal stress affected different genes in a similar fashion; represented as percentage in the table. For calculations the two copies of the Tos17 were treated separately

ISSN: 1471-2229