Fig. 1

Alteration in the steady-state transcript abundance determined by semi-quantitative RT-PCR analysis in a set of 18 randomly selected genes, which include two transposable element genes (Tos17 and Osr42), seven cellular genes (homeobox gene, DNA-binding protein, Elongation factor, HSP70, SNF-FZ14, S3, and YF25), and nine rice Heavy Metal-transporting P-type ATPases (OsHMA1-OsHMA9). The results were highly reproducible among the three independent RNA batches, and hence, only one was presented. Gene names are listed to the left and amplification cycles are labeled to the right of the gel. The rice Actin gene (Genbank accession # X79378) was used as a control for normalization of RNA input. Lack of genomic DNA was validated by the Actin gene on the template without RT