Fig. 9
From: Deposition of a cutin apoplastic barrier separating seed maternal and zygotic tissues
De novo cutin deposition in the IAB is triggered by central cell fertilization. a to d Fluorescence images of longitudinal sections of seeds and emasculated ovules, stained with auramine O (yellow) and counterstained with calcofluor (cyan). (a) and (b) Wild type seeds. Ecotype Ws. (c) and (d) kpl endosperm-only seeds. The orange arrow indicates the unfertilized, expanded egg-cell. Ecotype Ws. e Fluorescence image of a ProATT1:YFP ovule at 4DAE. Yellow, YFP; red, propidium iodide. Ecotype Col. f Fluorescence image of a progeny endosperm-only seed at 4DAP from a ProATT1:YFP (col) x kpl (Ws) cross. Yellow, YFP; red, propidium iodide. Scale bars: (a) to (d) and (f) 50 μm, (e) 30 μm