Fig. 2

IAB de novo cutin deposition after fertilization. a Analyses of ovule (0 DAF) and seed (4 and 8 DAF) fatty acyl composition by GC-MS. Values are relative to all detected fatty acyl chains. b RT-qPCR analyses of genes involved in cutin deposition in wild type seeds at 2 and 4 DAF. Values are relative to wild type ovules at 0 DAF. c to e YFP fluorescence (yellow) and auto-fluorescence (purple) images of ProATT1:YFP ovules (stage 3-VI) (c) and seeds at 2 cells (d) and late globular (e) embryo stages. f and g GUS activity in cleared whole mounts of a ProMYB30:gMYB30-uidA ovule (stage 3-VI) (f) and seed right after fertilization (g). WT, wild type. Error bars represent standard deviations. Asterisks indicate statistical difference between different time points (Student’s t test, *: P < 0.05; **: P < 0.01; ***: P < 0.001). The complete statistical analysis of (a) is in Additional file 1: Table S3. Ecotype Col. Scale bars: (c), (d), (f) and (g) 30 μm, (e) 50 μm