Fig. 6

Effect of FtMYB8 on the accumulation of anthocyanin/PA, gene expression and development of marginal trichomes. a Photographs of WT and transgenic seedlings grown in MS medium with 3% sucrose. (B-1) Mature Arabidopsis seeds. (B-2) PA accumulation in mature seeds stained by vanillin-HCl. c,d Anthocyanin/PA content in transgenic lines and WT plants. e Each sample used for total RNA extraction contained at least 50 seedlings. The mRNA abundances of ten genes were monitored by qRT-PCR in the WT and transgenic lines. The relative expression was evaluated by the 2^−ΔΔCT method, and gene expression levels were defined as “1” in WT Arabidopsis seedlings. Arabidopsis Atactin2 was used as a reference gene. f Comparison of trichome distribution between transgenic plants and WT plants at the true leaf stage by stereomicroscopy. g Total number of trichomes at the true leaf stage in the WT and transgenic lines. Each sample used to count the total number of trichomes contained at least 15 leaves. h Percentage of marginal trichomes in true leaves in the WT and transgenic lines. Each sample used to calculate the percentage of marginal trichomes contained at least 15 leaves. Means were calculated from three repeats and error bars reflect ±SDs. *P < 0.05, **P < 0.01