Fig. 2
BiFC assays in epidermis cells of tobacco leaf to identify the interactions between PCNA and RFC subunits of Arabidopsis and rice. a and b No YFP signal is detected between AtPCNA1-YFPC and YFPN/AtRFC1-YFPN. c-f AtPCNA1 can interact with AtRFC2/3/4/5, respectively. g and h No YFP signal is detected between AtPCNA2-YFPC and YFPN/AtRFC1-YFPN. i-l AtPCNA2 can interact with AtRFC2/3/4/5, respectively. m No YFP signal is detected between OsPCNA-YFPC and YFPN. n-r OsPCNA can directly interact with OsRFC1/2/3/4/5. The tobacco epidermal cells are co-transfected with constructs encoding the candidate fusion proteins. YFPC, the C-terminal fragment of YFP (156–239 aa); YFPN, the N-terminal fragment of YFP (1–155 aa). Bars = 50 μm