CRISPR/Cas9 editing of three CRUCIFERIN C homoeologues alters the seed protein profile in Camelina sativa

BMC Plant Biology

Table 2 Knockout of CsCRUC does not alter seed weight, protein or oil content

Line	100 seed weight (mg)^a	protein content (mg/g dry seed)^b	protein content of defatted meal (% N based)^b	oil content (mg/g dry seed)^c
wild type	114.3 ± 1.7	289.24 ± 9.30	44.01 ± 0.29	355.33 ± 5.08
CsCRUC ^{−/−,−/−,−/−}	105.7 ± 4.9	306.45 ± 30.24	44.48 ± 0.05	364.40 ± 4.31

^aSeed weight means are from three samples of seed from each of eight biological replicates
^bProtein analysis means are from three samples of seed from each of three biological replicates
^cOil analysis means are from one sample of seed from each of five biological replicates
^a-cMeans of wild-type and CsCRUC^{−/−,−/−,−/−} are not significantly different (p < 0.05, Student’s t-test). Means are shown ± S.E.M

ISSN: 1471-2229