Fig. 5
From: Modification of Threonine-1050 of SlBRI1 regulates BR Signalling and increases fruit yield of tomato
Dephosphorylation of Thr-1050 influences SlBRI1 autophosphorylation. a Autophosphorylation level of SlBRI1 in vitro. Autophosphorylation activity of recombinant FLAG-SlBRI1, FLAG-T1050A, FLAG-T1050D, and FLAG-K916E proteins was detected using anti-pThr antibodies, and aliquots of the recombinant proteins were detected using anti-FLAG antibodies and western blotting. Coomassie brilliant blue (CBB) staining shows loading. b The relative autophosphorylation levels of FLAG-SlBRI1, FLAG-T1050A, FLAG-T1050D, and FLAG-K916E proteins in vitro. The autophosphorylation level of FLAG-SlBRI1 was defined as “1”. Data are the means ± SDs of 3 independent measurements. c Autophosphorylation level of SlBRI1 in vivo. Autophosphorylation activity of SlBRI1-green fluorescent protein (GFP), T1050A-GFP, T1050D-GFP, and K916E-GFP proteins was detected using anti-pThr antibodies; anti-GFP antibodies were used to show the loading levels for western blotting. d The relative autophosphorylation levels of SlBRI1-GFP, T1050A-GFP, T1050D-GFP, and K916E-GFP proteins in vivo. The autophosphorylation level of SlBRI1-GFP was defined as “1”. Data are the means ± SDs of 3 independent measurements