Fig. 2

Development-defect phenotypes of the ppr287 mutants. a Schematic representation of T-DNA insertion sites and amiRNA knockdown mutant (KD1) target site. Black rectangles and lines represent exons and introns, respectively, and untranslated regions are indicated by white rectangles. The positions of T-DNA insertion in the ppr287 mutants and amiR target site are indicated by black and white triangles, respectively. The number of amino acid (aa) in PPR287 is indicated. Positions of primers used for RT-PCR in (b and c) are shown with arrowheads. b RT-PCR analysis confirming the absence or downregulation of PPR287 expression in the ppr287 mutant, amiR mutant (KD1), and complementation line (Com1) in ppr287 mutant background. The primer pair (1 + 2) amplifying the PPR287 transcript spanning the T-DNA insertion site was used. c RT-PCR (left) and quantitative real-time RT-PCR (right) analyses confirming the downregulation of PPR287 expression in the ppr287 mutant. The primer pair (3 + 4) amplifying the PPR287 transcript upstream of the T-DNA insertion site was used. Tubulin was used as a loading control. d Seed development of the heterozygous mutant. White arrows indicate aborted seeds in the mutants. Bar = 1 cm. e Pale-green phenotypes of the ppr287 and KD1 line observed at 6 days after germination