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Fig. 7 | BMC Plant Biology

Fig. 7

From: Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

Fig. 7

Truncations of Pol1A and Pol1B interacting with residues 120–295 of Twinkle. Truncations are numbered 1–12 and span Pol1A and Pol1B as designated above. Each truncation was tested for association with the interacting region of Twinkle and plated in 1:5 spot dilutions on selective and non-selective media as a control. Non-selective media is SD -leu -trp and the selective media is SD -leu -trp -his -ade. Note the stronger interaction between Pol1A and Twinkle versus Pol1B and Twinkle

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