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Fig. 2 | BMC Plant Biology

Fig. 2

From: Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

Fig. 2

Heat map of residues most likely to interact based on Direct Coupling Analysis. (a) Heat map showing the likelihood of residues from Twinkle (y-axis) to interact with residues from Pol1A (x-axis) based on mutual information generated from DCA. This heatmap was generated using multiple sequence alignment data of Twinkle, Pol1A and Pol1B homologs. The final heatmap can be drawn using either Pol1A or Pol1B on the x-axis with near identical results. This heatmap displays Pol1A on the x-axis. The raw data used to make this heat map was analyzed to identify 10 residues in both the polymerases and Twinkle with the highest likelihood for interaction. (b) Location of the 10 residues identified from DCA analysis mapped to Pol1A, Pol1B, and Twinkle (vertical red lines). These residues were picked for point mutation analysis to see how they would affect the Polymerase-Twinkle interaction

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BMC Plant Biology

ISSN: 1471-2229

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