Table 1 Kinetic parameters of SrSpmHT with fixed concentrations of spermine and varying acyl donor substrates
From: A novel hydroxycinnamoyl transferase for synthesis of hydroxycinnamoyl spermine conjugates in plants
Acyl donor | Vmax (nmol•s−1•mg− 1) | Km (μM) | kcat (s− 1) | kcat/Km (s− 1 μM− 1) |
|---|---|---|---|---|
caffeoyl-CoA | 1146.1 ± 101.4a | 478.4 ± 46.5a | 71.5 ± 6.3a | 0.150 ± 0.001b |
feruloyl-CoA | 223.0 ± 8.0b | 106.1 ± 9.0b | 13.9 ± 0.5b | 0.132 ± 0.006c |
ρ-coumaroyl-CoA | 135.8 ± 3.4c | 49.0 ± 1.2c | 8.5 ± 0.21c | 0.173 ± 0.001a |