Fig. 3

Subcellular localization and functional complementation analysis of TaAMT2;3a. a Subcellular localization of TaAMT2;3a in wheat protoplasts. Wheat protoplasts were transformed with a plasmid carrying a eGFP-TaAMT2;3a fusion protein. The picture was obtained using a fluorescence microscope. The top four images indicate GFP (control), and the bottom four images indicate eGFP-TaAMT2;3a. Bar: 10 μm. b Functional complementation analysis of TaAMT2;3a in yeast ammonium transporter mutant. Yeast mutant strain 31019b (mep1, mep2, mep3, ura3) cells were transformed with a pDR195 vector expressing TaAMT2;3a and tested for growth on media supplemented with 1 mM NH₄Cl, 5 mM NH₄Cl and 1 mM arginine (Arg). The empty vector (pDR195) was used as the control