Fig. 2

Multiple sequence alignment and phylogenetic analysis of TaAMT2;3a. a Multiple sequence alignment of amino acid sequences from Arabidopsis thaliana (AtAMT1;1, AtAMT2;1), Oryza sativa (OsAMT1;1, OsAMT2;1) and Triticum aestivum (TaAMT2;3a). Ammonium transporter domains: red line. CTR: black line. Highly conserved domain: blue linear box. b Phylogenetic analysis of TaAMT2;3a and homologues in wheat and other plant species. At: Arabidopsis thaliana; Ta: Triticum aestivum; Mp: Marchantia polymorpha; and Os: Oryza sativa. Multiple sequence alignments and the neighbour joining tree were created using the ClustalW algorithm by MEGA (version 7.0)