Fig. 1

Relative ammonium contents in different wheat tissues after inoculation with Puccinia striiformis f.sp. tritici (Pst) and the expression pattern of TaAMT2:3a. a Relative ammonium concentrations in leaves, stems, and roots from wheats inoculated with Pst at different time points. The relative ammonium concentration was calculated by subtracting the ammonium concentration of the non-inoculated control plants from that of the inoculated plants. b Relative transcription levels of the TaAMT2;3a gene in wheat leaves inoculated with Pst compared with that in the control plants. c The expression pattern of the TaAMT2;3a gene in different tissues. The data were normalized to the wheat TaEF-1a gene. All the results were obtained from three independent replicates. Asterisks indicate significant differences from 0 hpi using Student’s t-test. (P < 0.05)