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Fig. 2 | BMC Plant Biology

Fig. 2

From: The tomato WV gene encoding a thioredoxin protein is essential for chloroplast development at low temperature and high light intensity

Fig. 2

Ultrastructure of chloroplasts observation, protein analysis by SDS-PAGE, coomassie blue staining and immunoblot analysis in wv mutant and AC plants at 16 °C. a, b Chloroplasts in immature and mature leaves of AC plants, respectively. c, d Chloroplasts in immature and mature leaves of LA1526 plants, respectively. Cp, Chloroplast; St, Stroma; SG, starch grain; Thy, Thylakoid. e Protein analysis by SDS-PAGE and detection by coomassie blue staining. f Immunoblot analysis of chloroplast proteins in wv mutant. These proteins were PsaD (the photosystem I subunits); PsbA (photosystem II reaction center subunit); rbcL (the large subunit of Rubisco enzyme). The anti-actin served as a loading control. Magnifications of a, b, c and d are the same. Bar = 0.5 μm

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