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Table 3 Allelic diversity of thirty used EST-SSR markers in ninety-five accessions of Elymus

From: EST-SSR marker development based on RNA-sequencing of E. sibiricus and its application for phylogenetic relationships analysis of seventeen Elymus species

Primer

SSRs

Forward primer

Reverse primer

Tm (°C)

T

M

TP

PP

He

Ho

PIC

c11036

(TAG)5

CACTTGGGTGTGCAAAAGAA

TTCCCCTAGCAGGCAGATTA

58

5

0

5

100.0%

0.73

0.90

0.32

c24738

(AGC)5

ATTTTGGCTATTGAGCGTGG

GAAGATGGGCGACAAATTCT

58

22

0

22

100.0%

0.94

0.92

0.36

c40721

(GAG)5

GAGGGTAATGACCGTCTGGA

TCTGGCCATGTTGTTGTTGT

58

21

0

21

100.0%

0.94

0.97

0.39

c40810

(ACA)6

ATCTTCTATCACGGCCAACG

TTGATGGCACATTGAGATCC

58

15

0

15

100.0%

0.91

0.92

0.35

c45646

(TTG)5

CTGATGATGATGGCCAGTTG

GAGAGGTCATTCCGCATGTT

60

19

0

19

100.0%

0.94

0.96

0.42

c47765

(GCT)5

GAGAGAGACACACGGAAGGC

GACAGAGAGCTCGCAGGAG

62

23

0

23

100.0%

0.94

1.00

0.36

c56077

(TGA)5

GGCCAATCAAATTGGAACAT

AAGGCCTCCGAGCTAAAAAG

56

13

0

13

100.0%

0.91

0.89

0.37

c57159

(TGT)5

CGCCGCTTATCACTTTTGTT

ACTAATGATGTCGCCGATCC

58

21

0

21

100.0%

0.93

1.00

0.35

c60959

(ACT)5

CATTGTTGGCGTTCAATCAC

CCGTTAGCTCATCCAAGCTC

58

15

0

15

100.0%

0.91

0.96

0.38

c61134

(TCC)6

CCTCCCAGGTGACACGTACT

GGTAGGGGGCGTAAGAAGAG

64

11

0

11

100.0%

0.85

1.00

0.28

c62352

(CGG)6

TTTTAGAGCAGCAGCAGCAA

GACCCGGAGAAGATCAACAA

58

22

0

22

100.0%

0.93

1.00

0.32

c64785

(CTT)5

TCCCTTTGTCTCCCTCTCCT

ACAATCACGGCGATAAGGTC

60

18

0

18

100.0%

0.92

0.94

0.33

c65566

(CGC)5

TCCTCGTCTCCTCCCTCC

CTCGTCCTCGTAGTGCTCCT

60

23

0

23

100.0%

0.95

1.00

0.41

c66150

(CGG)5

CTCAGATCGTCCTCCGTCAC

CTCCTCCTGCTGCTCGTC

60

25

0

25

100.0%

0.95

1.00

0.34

c66252

(CTT)6

GGAGGAGGAGAACTTCCTGG

TCACCGGAAAAACACATTCA

56

21

0

21

100.0%

0.94

1.00

0.33

c66352

(CTG)5

GACTACTGGAGGCGGACTTG

GAGAGCAGGCAGAGAGCCTA

64

19

0

19

100.0%

0.93

1.00

0.31

c66859

(AAG)5

AGCGACCTGGACATGAACTC

GAATCCACCCTGAAGGATCA

60

20

0

20

100.0%

0.94

0.96

0.41

c67170

(CCG)5

GGACCTCCCACAAAGTGAAA

AGTAGCTCCCCACCACGAG

60

22

0

22

100.0%

0.94

0.99

0.38

c67290

(CGG)5

AGACGAGGACGTCGGTGTT

AGACACGCGAGAGGGTAGAC

60

25

0

25

100.0%

0.95

1.00

0.41

c68346

(AGC)5

CGAGTTGTGTCACCCAGAGA

ACAAGCAAGGCATACCCAAG

60

16

0

16

100.0%

0.92

1.00

0.37

c68713

(CGG)6

GTTCGTCGTCTCCGCATC

AAGAAGCACAGGCCAAGC

56

25

0

25

100.0%

0.95

1.00

0.43

c69557

(GCG)5

ACCAGGCGATTTATGGACAG

GTGACCGCAACAATGACAAC

60

19

0

19

100.0%

0.94

0.95

0.38

c69822

(GCA)5

CAGCCGAAAATTTCCGTAGA

GCCATTTGAGGAGGACAAAA

58

20

0

20

100.0%

0.93

1.00

0.28

c70441

(CGG)5

TGGAGGAGACGGTAGAGGTG

CAAGAATAAACGGGAGCGAA

58

21

0

21

100.0%

0.93

1.00

0.33

c70704

(AAG)5

ACCTCAACCGTGCTCTCAAG

TCCTTGGCCATCAACTTCTC

60

19

0

19

100.0%

0.94

1.00

0.40

c70817

(GAC)5

AGCCATGCTCAGGACCATAC

TACATTCTGCTGCTTGGCAC

60

17

0

17

100.0%

0.93

0.99

0.41

c71552

(GCC)5

CACTTCTCTCTCTCCCTCGC

CCGTGGATGACAACGGTTAC

62

19

0

19

100.0%

0.93

1.00

0.36

c75219

(GGA)5

CACTCGCAACCGAGGAAG

CGGAGTCCAACGTCGTCTAC

58

16

0

16

100.0%

0.92

1.00

0.38

c79438

(TAA)5

TGAATGATTCTCGCGAAGTG

GAGGAGGGCAAACAACAAAA

58

20

0

20

100.0%

0.93

0.99

0.36

c82957

(GCC)6

GAGAACAACGGGCAAGAAGA

GGGACGATTTGGAACAGCTA

60

20

0

20

100.0%

0.94

0.98

0.40

Mean

    

19.1

0

19.1

100.0%

0.92

0.98

0.36

  1. T total number of amplified bands, M number of monomorphic bands, TP total number of polymorphic bands, PP percentage of polymorphism, He expected heterozygosity, Ho observed heterozygosity, PIC polymorphic information content