|
Primer
|
SSRs
|
Forward primer
|
Reverse primer
|
Tm (°C)
|
T
|
M
|
TP
|
PP
|
He
|
Ho
|
PIC
|
|---|
|
c11036
|
(TAG)5
|
CACTTGGGTGTGCAAAAGAA
|
TTCCCCTAGCAGGCAGATTA
|
58
|
5
|
0
|
5
|
100.0%
|
0.73
|
0.90
|
0.32
|
|
c24738
|
(AGC)5
|
ATTTTGGCTATTGAGCGTGG
|
GAAGATGGGCGACAAATTCT
|
58
|
22
|
0
|
22
|
100.0%
|
0.94
|
0.92
|
0.36
|
|
c40721
|
(GAG)5
|
GAGGGTAATGACCGTCTGGA
|
TCTGGCCATGTTGTTGTTGT
|
58
|
21
|
0
|
21
|
100.0%
|
0.94
|
0.97
|
0.39
|
|
c40810
|
(ACA)6
|
ATCTTCTATCACGGCCAACG
|
TTGATGGCACATTGAGATCC
|
58
|
15
|
0
|
15
|
100.0%
|
0.91
|
0.92
|
0.35
|
|
c45646
|
(TTG)5
|
CTGATGATGATGGCCAGTTG
|
GAGAGGTCATTCCGCATGTT
|
60
|
19
|
0
|
19
|
100.0%
|
0.94
|
0.96
|
0.42
|
|
c47765
|
(GCT)5
|
GAGAGAGACACACGGAAGGC
|
GACAGAGAGCTCGCAGGAG
|
62
|
23
|
0
|
23
|
100.0%
|
0.94
|
1.00
|
0.36
|
|
c56077
|
(TGA)5
|
GGCCAATCAAATTGGAACAT
|
AAGGCCTCCGAGCTAAAAAG
|
56
|
13
|
0
|
13
|
100.0%
|
0.91
|
0.89
|
0.37
|
|
c57159
|
(TGT)5
|
CGCCGCTTATCACTTTTGTT
|
ACTAATGATGTCGCCGATCC
|
58
|
21
|
0
|
21
|
100.0%
|
0.93
|
1.00
|
0.35
|
|
c60959
|
(ACT)5
|
CATTGTTGGCGTTCAATCAC
|
CCGTTAGCTCATCCAAGCTC
|
58
|
15
|
0
|
15
|
100.0%
|
0.91
|
0.96
|
0.38
|
|
c61134
|
(TCC)6
|
CCTCCCAGGTGACACGTACT
|
GGTAGGGGGCGTAAGAAGAG
|
64
|
11
|
0
|
11
|
100.0%
|
0.85
|
1.00
|
0.28
|
|
c62352
|
(CGG)6
|
TTTTAGAGCAGCAGCAGCAA
|
GACCCGGAGAAGATCAACAA
|
58
|
22
|
0
|
22
|
100.0%
|
0.93
|
1.00
|
0.32
|
|
c64785
|
(CTT)5
|
TCCCTTTGTCTCCCTCTCCT
|
ACAATCACGGCGATAAGGTC
|
60
|
18
|
0
|
18
|
100.0%
|
0.92
|
0.94
|
0.33
|
|
c65566
|
(CGC)5
|
TCCTCGTCTCCTCCCTCC
|
CTCGTCCTCGTAGTGCTCCT
|
60
|
23
|
0
|
23
|
100.0%
|
0.95
|
1.00
|
0.41
|
|
c66150
|
(CGG)5
|
CTCAGATCGTCCTCCGTCAC
|
CTCCTCCTGCTGCTCGTC
|
60
|
25
|
0
|
25
|
100.0%
|
0.95
|
1.00
|
0.34
|
|
c66252
|
(CTT)6
|
GGAGGAGGAGAACTTCCTGG
|
TCACCGGAAAAACACATTCA
|
56
|
21
|
0
|
21
|
100.0%
|
0.94
|
1.00
|
0.33
|
|
c66352
|
(CTG)5
|
GACTACTGGAGGCGGACTTG
|
GAGAGCAGGCAGAGAGCCTA
|
64
|
19
|
0
|
19
|
100.0%
|
0.93
|
1.00
|
0.31
|
|
c66859
|
(AAG)5
|
AGCGACCTGGACATGAACTC
|
GAATCCACCCTGAAGGATCA
|
60
|
20
|
0
|
20
|
100.0%
|
0.94
|
0.96
|
0.41
|
|
c67170
|
(CCG)5
|
GGACCTCCCACAAAGTGAAA
|
AGTAGCTCCCCACCACGAG
|
60
|
22
|
0
|
22
|
100.0%
|
0.94
|
0.99
|
0.38
|
|
c67290
|
(CGG)5
|
AGACGAGGACGTCGGTGTT
|
AGACACGCGAGAGGGTAGAC
|
60
|
25
|
0
|
25
|
100.0%
|
0.95
|
1.00
|
0.41
|
|
c68346
|
(AGC)5
|
CGAGTTGTGTCACCCAGAGA
|
ACAAGCAAGGCATACCCAAG
|
60
|
16
|
0
|
16
|
100.0%
|
0.92
|
1.00
|
0.37
|
|
c68713
|
(CGG)6
|
GTTCGTCGTCTCCGCATC
|
AAGAAGCACAGGCCAAGC
|
56
|
25
|
0
|
25
|
100.0%
|
0.95
|
1.00
|
0.43
|
|
c69557
|
(GCG)5
|
ACCAGGCGATTTATGGACAG
|
GTGACCGCAACAATGACAAC
|
60
|
19
|
0
|
19
|
100.0%
|
0.94
|
0.95
|
0.38
|
|
c69822
|
(GCA)5
|
CAGCCGAAAATTTCCGTAGA
|
GCCATTTGAGGAGGACAAAA
|
58
|
20
|
0
|
20
|
100.0%
|
0.93
|
1.00
|
0.28
|
|
c70441
|
(CGG)5
|
TGGAGGAGACGGTAGAGGTG
|
CAAGAATAAACGGGAGCGAA
|
58
|
21
|
0
|
21
|
100.0%
|
0.93
|
1.00
|
0.33
|
|
c70704
|
(AAG)5
|
ACCTCAACCGTGCTCTCAAG
|
TCCTTGGCCATCAACTTCTC
|
60
|
19
|
0
|
19
|
100.0%
|
0.94
|
1.00
|
0.40
|
|
c70817
|
(GAC)5
|
AGCCATGCTCAGGACCATAC
|
TACATTCTGCTGCTTGGCAC
|
60
|
17
|
0
|
17
|
100.0%
|
0.93
|
0.99
|
0.41
|
|
c71552
|
(GCC)5
|
CACTTCTCTCTCTCCCTCGC
|
CCGTGGATGACAACGGTTAC
|
62
|
19
|
0
|
19
|
100.0%
|
0.93
|
1.00
|
0.36
|
|
c75219
|
(GGA)5
|
CACTCGCAACCGAGGAAG
|
CGGAGTCCAACGTCGTCTAC
|
58
|
16
|
0
|
16
|
100.0%
|
0.92
|
1.00
|
0.38
|
|
c79438
|
(TAA)5
|
TGAATGATTCTCGCGAAGTG
|
GAGGAGGGCAAACAACAAAA
|
58
|
20
|
0
|
20
|
100.0%
|
0.93
|
0.99
|
0.36
|
|
c82957
|
(GCC)6
|
GAGAACAACGGGCAAGAAGA
|
GGGACGATTTGGAACAGCTA
|
60
|
20
|
0
|
20
|
100.0%
|
0.94
|
0.98
|
0.40
|
|
Mean
| | | | |
19.1
|
0
|
19.1
|
100.0%
|
0.92
|
0.98
|
0.36
|
- T total number of amplified bands, M number of monomorphic bands, TP total number of polymorphic bands, PP percentage of polymorphism, He expected heterozygosity, Ho observed heterozygosity, PIC polymorphic information content
