Fig. 6

Relative expression levels of key genes involved in chilling, heat and ROS signaling. qPCR analysis of CBF1 (a), MYB83 (b), HsfA1a (c), HsfA1b (d), Hsp90 (e), CuZnSOD (f), FeSOD (g), CAT (h), APX1 (i), APX2 (j), ZAT12 (k) and ZAT10 (l) in sha-miR319d-overexpressing lines and WT. T2 generations of transgenic plants as well as WT were treated with chilling or heat for 0 (control), 4 and 8 h. The reference gene was Actin. Each value is the mean of three biological repeats ± the standard deviation (SD). Asterisks indicate significant differences between WT and transgenic plants. *, P < 0.05; **, P < 0.01, Student’s t test.