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Fig. 4 | BMC Plant Biology

Fig. 4

From: Overexpression of Solanum habrochaites microRNA319d (sha-miR319d) confers chilling and heat stress tolerance in tomato (S. lycopersicum)

Fig. 4

The effect of overexpression of sha-miR319d on ROS accumulation. NBT staining for O2− in the leaves of WT and transgenic plants under control conditions and after 1 d of chilling (4 °C) or heat (40 °C) treatment. b DAB staining for H2O2 in the leaves of WT and transgenic plants under control conditions and after 1 d of chilling (4 °C) or heat (40 °C) treatment. c Quantitative analysis of O2− in the leaves of WT and transgenic plants under control conditions and after 1 d of chilling (4 °C) or heat (40 °C) treatment. d Quantitative analysis of H2O2 in the leaves of WT and transgenic plants under control conditions and after 1 d of chilling (4 °C) or heat (40 °C) treatment. T2 generations of transgenic plants as well as WT were used for O2− and H2O2 histochemical staining and quantitative analysis. Each value is the mean of three biological repeats ± the standard deviation (SD). Asterisks indicate significant differences between transgenic lines and WT. *, P < 0.05; **, P < 0.01, Student’s t test. The bar in (a) and (b) indicates 1 cm

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