Fig. 1From: Overexpression of Solanum habrochaites microRNA319d (sha-miR319d) confers chilling and heat stress tolerance in tomato (S. lycopersicum)Identification of sha-miR319d overexpression transgenic plants. a Genomic PCR analysis of sha-MIR319d in WT and transgenic plants. b Expression levels of sha-miR319d in WT and T2 generations of transgenic plants. The reference gene for sha-miR319d was U6snRNA. Each value was the mean of three biological repeats ± the standard deviation (SD). c Leaf morphogenesis of WT and transgenic lines (T1 generations). d, e The plant height of 30-day-old WT and transgenic lines (T2 generations). Asterisks indicate significant differences between WT and transgenic plants. *, P < 0.05; **, P < 0.01, Student’s t testBack to article page