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Table 2 Proteins and their fold changes in the leaves and roots of stylo under Mn toxicity

From: Physiological responses and proteomic changes reveal insights into Stylosanthes response to manganese toxicity

Accession number (a)

Protein name (b)

Protein abbreviation

Fold change

Leaf (Mn/CK)

Root (Mn/CK)

Photosynthesis

I1LC20

Chlorophyll a-b binding protein

LHCa/b1

n.s.

Suppressed

A0A151RZX1

Chlorophyll a-b binding protein

LHCa/b2

0.46

n.s.

A0A1S3TRJ8

Chlorophyll a-b binding protein

LHCa/b3

0.33

n.s.

A0A1L5BWG5

Photosystem I P700 chlorophyll a apoprotein A1

PsaA

0.46

n.s.

V5JDR5

Photosystem I iron-sulfur center

PsaC

0.49

n.s.

D3J8F9

Photosystem II CP43 reaction center protein

PsbC1

0.29

n.s.

R9ZUR7

Photosystem II CP43 reaction center protein

PsbC2

Induced

n.s.

G7K9H5

Photosystem II oxygen-evolving enhancer protein

OEE

n.s.

Suppressed

A0A1S2XT02

Photosynthetic NDH subunit of subcomplex B 2

PNSB2

Suppressed

n.s.

A0A1S2YJB0

Electron transfer flavoprotein subunit alpha

ETFA

Suppressed

n.s.

Q9LKH8

NADPH-protochlorophyllide oxidoreductase

Por

n.s.

Suppressed

A0A0B2QNM0

ATP-dependent Clp protease ATP-binding

subunit clpA like CD4B

CD4B

n.s.

Suppressed

V7BKQ7

Cytochrome c oxidase subunit 5C

COX5C

2.34

n.s.

E3NYU2

Rubisco activase 2

RCA

0.30

n.s.

E0D980

Ribulose bisphosphate carboxylase large chain

rbcL

0.28

n.s.

A0A0R0FCD1

Phosphoglycolate phosphatase

PGLP

0.50

n.s.

A0A072V9Z1

Peroxisomal (S)-2-hydroxy-acid oxidase GLO1

GLO

n.s.

Induced

A0A1J7GY35

Serine hydroxymethyltransferase

SHMT

0.42

n.s.

A0A151T1I8

Serine-glyoxylate aminotransferase

SGAT

n.s.

Induced

TCA cycle

A0A0B2S2G8

Citrate synthase

CS

n.s.

2.04

A0A1S3VRH0

Succinate dehydrogenase

SDH

Induced

n.s.

G7JYQ8

Aconitate hydratase

AH1

Induced

n.s.

A0A1S2Z8R6

Aconitate hydratase

AH2

n.s.

0.47

Carbon fixation

C9W980

Phosphoenolpyruvate carboxylase

PEPC1

2.58

n.s.

B0LXE5

Phosphoenolpyruvate carboxylase

PEPC2

2.75

n.s.

Q8H946

Phosphoenolpyruvate carboxylase

PEPC3

3.80

n.s.

Q8W4X0

Cytosolic malate dehydrogenase

MDH

Suppressed

n.s.

A0A0L9VHL1

Malic enzyme

ME

n.s.

Suppressed

Glycolysis

I1M6D5

Fructose-bisphosphate aldolase

FBPA1

n.s.

Induced

I3SUU7

Fructose-bisphosphate aldolase

FBPA2

Induced

n.s.

V7C1L3

Glucose-6-phosphate isomerase

G6PI

Suppressed

n.s.

Amino acid metabolism

O04998

Glutamine synthetase

GLN

2.49

n.s.

A0A1S3VJ34

Glutamate synthase

GLT

n.s.

0.33

A0A142F3D2

Glutamate dehydrogenase

GluDH

Induced

n.s.

A0A1S3TXT3

Ornithine carbamoyltransferase

OCT

n.s.

Induced

A0A1S3EC55

S-adenosylmethionine synthase

SAMS

2.37

n.s.

Other metabolism

A0A0B2PML9

Sucrose synthase 2

SuSy

Suppressed

n.s.

A0A151TQQ3

Alde 1-epimerase

Aepi

4.18

n.s.

Q7X9T1

Alpha-amylase

AMY

Induced

n.s.

H6U596

Alcohol dehydrogenase

ADH

Induced

n.s.

B0FBK6

Acetolactate synthase

ALS

n.s.

Induced

A0A0B2NWN8

3-hydroxyisobutyryl-CoA hydrolase-like protein 5

3-HCHL

Suppressed

n.s.

A0A151R9N4

Formate dehydrogenase

FDH

Induced

n.s.

ATP synthase

A0A1J7HQT2

ATP synthase subunit beta

ATP synthase1

n.s.

Induced

A0A1S2YAS0

ATP synthase subunit b

ATP synthase2

n.s.

Suppressed

A0A072TW18

F0F1-type ATP synthase

ATP synthase3

Induced

n.s.

A0A120IH31

ATPase subunit 8

ATPase

Induced

n.s.

Defense response

A0A1J7HRM7

Peroxidase

POD1

2.95

n.s.

P22195

Cationic peroxidase 1

POD2

2.18

n.s.

A0A072UXA0

Peroxidase

POD3

Induced

n.s.

A0A0B2SVW0

Peroxidase

POD4

Induced

n.s.

A0A1J7G991

Peroxidase

POD5

Induced

n.s.

A0A151TL42

Peroxidase

POD6

Induced

n.s.

G7INV1

Peroxidase

POD7

Induced

n.s.

A0A151R770

Peroxidase

POD8

n.s.

0.45

K7KG78

Peroxidase

POD9

n.s.

2.70

I1LI46

Peroxidase

POD10

n.s.

Suppressed

A0A1S2Y825

Peroxidase

POD11

n.s.

Suppressed

A0A1S3T7B9

Peroxidase

POD12

n.s.

Suppressed

A0A1S2YK26

Probable glutathione S-transferase

GST

3.75

n.s.

Q5XXQ4

Pathogenesis-related protein 10

PR101

n.s.

2.21

Q6VT83

Pathogenesis-related protein 10

PR102

n.s.

0.10

K4LMW7

Pathogenesis-related protein 10b

PR10b

Induced

n.s.

Q7X9F6

Chitinase class Ib

CHI

Induced

n.s.

A0A151R8U8

Endochitinase

ECHI

Induced

n.s.

Q9S9H7

Beta-1,3-glucanase

GLU

n.s.

0.27

A2Q4Q4

Polyphenol oxidase

PPO

2.33

n.s.

G7K6C8

NAD(P)H:quinone oxidoreductase

NQOR

Induced

n.s.

A0A1S3VW46

Quinone oxidoreductase-like protein 2 homolog

QOR

Induced

n.s.

A0A1S3VNB5

Probable NAD(P)H dehydrogenase (Quinone)

FQR1-like 2

FQR1L2

n.s.

Suppressed

A0A0K0K9R9

Universal stress protein

USP

Suppressed

Suppressed

G9C018

Cold dehydrin

CDD

Induced

n.s.

A0A151TJU2

Osmotin-like protein

Osmotin

Induced

3.46

A0A151T914

Light-regulated protein

LRP

0.37

n.s.

A0A151TUS7

Protein IN2–1 isogeny B

IN2–1

2.66

n.s.

Phenylpropanoid pathway

A0PBZ9

Phenylalanine ammonia-lyase

PAL1

Induced

n.s.

A0A0R4J2S3

Phenylalanine ammonia-lyase

PAL2

n.s.

Induced

Q01287

Chalcone synthase 2

CHS

n.s.

Induced

A0A1S2Y0E4

Chalcone-flavonone isomerase family protein

CFI

2.30

n.s.

A0A068JKQ1

Isoflavone reductase

IFR1

n.s.

0.20

A0A151TIC8

Isoflavone reductase

IFR2

n.s.

0.49

P52576

Isoflavone reductase

IFR3

2.71

n.s.

A0A072UCP6

Isoflavone reductase-like protein

IFRL

3.67

0.43

A0A1S2YI24

2-hydroxyisoflavanone synthase-like

IFS

n.s.

Suppressed

Lignin pathway

A0A1J7GGU7

Dirigent protein

DIR1

Induced

n.s.

A0A151SV45

Dirigent protein

DIR2

2.13

n.s.

A0A0L9VQD6

Dirigent protein

DIR3

n.s.

Induced

E3NYT4

Dirigent protein

DIR4

n.s.

2.36

Cytoskeleton

A0A072VQC8

Tubulin alpha chain

α-tubulin

n.s.

Induced

P37392

Tubulin beta-1 chain

β-tubulin

n.s.

Induced

Cell wall modulation

M1PNG4

Expansin

Exp

Suppressed

n.s.

G7IMV1

Alpha-L-arabinofuranosidase/beta-D-xylosidase

ASD/BXL

n.s.

3.73

A0A0B2PFS3

Fasciclin-like arabinogalactan protein 12

FLA

n.s.

0.50

A0A151SRS4

Xyloglucan endotransglucosylase/hydrolase

XTH

n.s.

2.01

A0A1S3UQ47

Plasmodesmata callose-binding protein 3

PDCB

Induced

2.17

Transcription

A0A0B2Q3X5

Histone H1.2

H1.2

n.s.

2.31

C6TDQ3

Nuclear pore complex protein NUP35

NUP35

Induced

n.s.

RNA processing

A0A151SA18

Ribonucleases P/MRP protein subunit POP1

POP1

n.s.

5.88

Protein synthesis

A0A1S2X984

Elongation factor 1-gamma

EF1g

Induced

Induced

A0A072VFI9

Elongation factor 1-alpha

EF1a

Suppressed

n.s.

V7ATK1

Eukaryotic translation initiation factor 3 subunit B

eTIF3b

Induced

n.s.

A0A1J7G0E8

Eukaryotic translation initiation factor 3 subunit G

eTIF3g

2.19

n.s.

P17092

30S ribosomal protein S17

S17

2.23

n.s.

N0DM61

30S ribosomal protein S7

S7

n.s.

Suppressed

A0A1S2Z5B3

50S ribosomal protein L4

L4

n.s.

0.46

A0A191UJA2

50S ribosomal protein L14

L14

n.s.

Suppressed

Protein processing and transporting

A0A1S2X9H9

Heat shock 70 kDa protein

HSP70

2.08

n.s.

G7IDZ4

Heat shock protein 81–2

HSP81–2

n.s.

2.29

I1L314

Heat shock protein 90–1

HSP90–1

n.s.

Induced

I1LD87

Protein disulfide isomerase-like 7

PDIL7

Induced

n.s.

A0A151T7Z7

UDP-glucose:glycoprotein glucosyltransferase 1

UGGT

2.56

n.s.

A0A1S2Z565

Dolichyl-diphosphooligosaccharide-protein

glycosyltransferase subunit STT3B

STT3B

Induced

n.s.

Protein degradation

A0A072TU80

Ubiquitin-conjugating enzyme

UBC

Induced

n.s.

A0A151TZ12

E3 ubiquitin-protein ligase UPL1

UPL1

Induced

n.s.

A0A1S2Z415

Leucine aminopeptidase 1-like

LAPL1

Suppressed

n.s.

Signaling

A0A1S3VLK4

CBS domain-containing protein CBSX1

CBSX1

Induced

n.s.

A0A072UP16

Osmosensor histidine kinase

OHK

n.s.

Induced

A0A151TSS1

14–3-3 like protein B

14–3-3

2.21

n.s.

A0A151SLL9

Putative inactive receptor kinase

IRK

Induced

n.s.

A0A0L9U108

Serine/threonine-protein phosphatase

PP

Suppressed

n.s.

A0A072UKS2

PfkB family carbohydrate kinase

PfkB

Suppressed

n.s.

G7JHI0

General regulatory factor 2

GRF2

Suppressed

n.s.

I3S111

RAB GTPase-like protein A1D

A1D

n.s.

Suppressed

G7L1E7

C2 calcium/lipid-binding and GRAM domain protein

CalBP

n.s.

Suppressed

  1. Note: aDatabase accession number from UniProt; bThe name and functional categories of the proteins identified by LC-MS/MS. Stylo seedlings were grown under normal conditions for 30 d and then treated with 5 (CK) or 400 μM (Mn) MnSO4 for 10 d. Differentially expressed proteins in the leaves and roots of RY5 regulated by Mn toxicity were identified using a label-free quantitative proteomics approach. n.s. represents no significant difference