Fig. 4

Interaction between PbrSLAH3 and PbrCPK32 as assessed by yeast two-hybrid and bimolecular fluorescence complementation assays. a Yeast strain NMY51 co-transformed with the PbrSLAH3 bait vector and PbrCPK32 prey vector, along with a positive control (co-transformed NubI vector and pDHB vector) were grown in medium lacking Ade, His, Trp and Leu. The negative control could not grow in the same medium. b YFP indicates yellow fluorescence protein; Chl: chlorophyll; Bright: bright-field image of Nicotiana benthamiana leaves infiltrated with Agrobacterium tumefaciens; Merge: digital merge of bright field and fluorescent images. Bar = 10 μm