Fig. 3

PbrSLAH3 rescues the ammonium toxicity of slah3–3 mutant plants under high ammonium/low-nitrate conditions. a Schematic map of the T-DNA insertion site in the slah3–3 mutant. b RT-PCR analyses of PbrSLAH3 and AtSLAH3 expression levels in transgenic, wild-type (Col-0) and slah3–3 mutant plants. Actin was used as a control. c Growth comparison among 7-d-old transgenic lines 1 and 2, wild-type (Col-0) and slah3–3 mutant seedlings grown on 1/2 N-free Murashige and Skoog medium supplemented with different concentrations of KNO₃ (0–20 mM) and 20 mM NH₄Cl. d–g The average primary root lengths of seedlings under the same conditions as displayed in (c) were statistical analyzed. Results are averages ± SEs of three independent experiments (n = 15 per experiment). The asterisk represents statistical significance among transgenic lines, wild-type and slah3–3 mutant plant (Student’s t-test, *P < 0.05). Bar = 0.5 cm