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Fig. 1 | BMC Plant Biology

Fig. 1

From: PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear

Fig. 1

The determination of S-type anion channels in pear and the expression analysis. a Phylogenetic tree of the Arabidopsis thaliana SLAC/SLAH anion channel family and the pear orthologs. Four putative PbrSLAC/SLAH anion channels were identified in the Pyrus genome database. PbrSLAC1 appears to be related to AtSLAC1, other three homologs were classified into the AtSLAH2/3 group, while AtSLAH1/4 homologs could not be identified in pear. The PbrSLAH2/3–1 sequence was used to clone the ortholog from Pyrus bretschneideri and named PbrSLAH3. b The expression patterns of PbrSLAC/SLAH family genes in the root, leaf, pollen grain and pollen tube of pear were analyzed by qRT-PCR. The values displayed in the figure were the geometric means of the relative expression of PbrSLAC/SLAH when both PbrTUB and PbrUBQ as reference genes. Three biological and three technical replicates were processed for the qRT-PCR assays. The error bars indicate standard deviations. The data are shown as mean values ± SDs. c RT-PCR was used to confirm the expression patterns of the PbrSLAH3 gene and PbrUBQ gene was used as an internal control

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