Fig. 7

Transcription factor analysis. a Comparison of transcription factors in T. cuspidata, A. thaliana, and S. miltiorrhiza. b. Multiple sequence alignments of the WRKY domains of Group IIa WRKY proteins in T. cuspidata. Conserved WRKY amino acid signatures are indicated by short red lines, and zinc finger motifs are indicated by short blue lines. c Multiple sequence alignments of bHLH domains from subgroup IIIe bHLH proteins in T. cuspidata. The locations of the basic, helix, and loop regions within the bHLH domain are indicated in the scheme. The conserved amino acid signature of the basic motifs and the helix–loop–helix motifs are indicated by short red and blue short lines, respectively. d Multiple sequence alignments of AP2 domains from Group B1 and B3 ERF proteins in T. cuspidata. The three β-sheet regions and the α-helix region are labeled. The conserved amino acid signatures of β-1, β-2, β-3 and α-helix are indicated by short red, blue, green and purple lines, respectively. The essential residues for GCC-box binding in TcERF12 and TcERF15 are indicated by stars (*). e Expression analysis of identified TFs and candidate unigenes in the Taxol pathway by real-time PCR