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Fig. 4 | BMC Plant Biology

Fig. 4

From: Molecular cytogenetic characterisation of Elytrigia ×mucronata, a natural hybrid of E. intermedia and E. repens (Triticeae, Poaceae)

Fig. 4

Mitotic metaphase chromosomes of heptaploid E. ×mucronata after sequential FISH and GISH experiments. a and c GISH in plants C25B and C9: Aegilops tauschii (digoxigenin, anti-DIG-FITC, yellow pseudocolour), Dasypyrum villosum (biotin, streptavidin-Cy3, blue pseudocolour), Hordeum bogdanii (biotin, streptavidin-Cy3, green pseudocolour), and Pseudoroegneria spicata (Cy5, red). b and d FISH in plants C25B and C9: 5S rDNA (Cy5, red), 18S rDNA (digoxigenin, anti-DIG-FITC, green), and pSc119.2 (Cy3, white pseudocolour – applied only in C9 – d). After FISH, chromosomes were counterstained with DAPI. Different types of structural chromosomal alterations are indicated with numerals (1, 3, 4, 6). The chromosomal segment broken from the Aegilops chromosome is connected by a dotted line (c and d). Scale bars = 10 μm

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BMC Plant Biology

ISSN: 1471-2229

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