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Fig. 1 | BMC Plant Biology

Fig. 1

From: The Solanum chacoense Fertilization-Related Kinase 3 (ScFRK3) is involved in male and female gametophyte development

Fig. 1

Phylogenic positioning of ScFRK3 inside the FRK class and cellular localization. a. Phylogenetic tree of the ScFRKs (ScFRK1–6) and AtMAPKKK13–21. ScFRK3 and ScFRK4 are the closest orthologs of the Arabidopsis MAPKKK19–21. The tree was made by the Neighbor Joining method using 1000 replicates and rooted with the kinase domain of AtBRI1. b. (i) Transient expression of ScFRK3-GFP in onion cells through microparticle bombardment showing the subcellular localization of ScFRK3 in the cytoplasm and nucleus. Nuclear localization confirmation of (ii) with Hoechst 33342 DNA stain. (iii) DIC image of (i and ii). (iv-vi) Negative control with (iv) bombardement of empty vector (pMDC83); (v) Nuclear localization of (iv) with Hoechst 33342 DNA stain; (vi) DIC image of v and vi. Arrowhead points to the nucleus. Scale bars: 50 μm

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