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Fig. 7 | BMC Plant Biology

Fig. 7

From: Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Fig. 7

The conversion from (S)-naringenin (1) to dihydrokaempferol (1a) catalysed by OcFLS1 (a-c) and OcFLS2 (d-f) in the presence of Fe2+. a, d: HPLC chromatogram of the reaction product of (S)-naringenin (1) with OcFLS1 (a) and OcFLS2 (d). a, co-elution of the reaction product with authentic dihydrokaempferol (1a). b, the reaction product of (S)-naringenin (1) with the purified protein. c, the reaction product of (S)-naringenin (1) without the purified protein. b, e: UV spectrum of reaction product 1a (blue line) and authentic dihydrokaempferol (red line). c, f: MS spectrum of reaction product 1a produced by OcFLS1 (c) and OcFLS2 (f)

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