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Fig. 3 | BMC Plant Biology

Fig. 3

From: Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq

Fig. 3

The bioconversion of kaempferol (1b) from dihydrokaempferol (1a) catalysed by OcFLS1 (a-c) or OcFLS2 (d-f). a, d: HPLC chromatogram of the reaction product of dihydrokaempferol (1a) with OcFLS1 (a) and OcFLS2 (d). a, co-elution of the reaction product with authentic kaempferol (1b). b, the reaction product of dihydrokaempferol (1a) with purified protein. c, the reaction product of dihydrokaempferol (1a) without purified protein. b, e: UV spectrum of reaction product 1b (blue line) and authentic kaempferol (red line). c, f: MS spectrum of reaction product 1b produced by OcFLS1 (c) and OcFLS2 (f)

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