Table 1 Analysis of variance (F-values; across all environments) for near-isogenic lines (NILs) in CDC Go and CDC Alsask backgrounds, carrying all combinations of three Fusarium head blight (FHB) resistance genes: Fhb1, Fhb2, and Fhb5. Fixed effects of FHB resistance genes and entry (nested within gene) are provided for FHB severity assessed in the greenhouse at 14 days post inoculation (GH14), 21 days post inoculation (GH21), area under disease progress curve (GH_AUDPC), field incidence (FLD_INC), field severity (FLD_SEV), field FHB index (FLD_IND), deoxynivalenol (FLD_DON) accumulation, and broad-sense heritability (H2). For greenhouse data, the effect of chemotype (3ADON or 15ADON), and chemotype by gene interaction is also presented
Effect | dfa | GH14 | GH21 | GH_AUDPC | FLD_INC | FLD_SEV | FLD_IND | FLD_DON |
|---|---|---|---|---|---|---|---|---|
CDC Alsask | ||||||||
Gene (G) | 12 | 82.47*** | 364.28* | 178.70*** | 9.60*** | 16.82*** | 13.25*** | 5.20*** |
Gene (entry) | 29 | 2.29ns | 3.42ns | 3.90*** | 1.86ns | 1.21ns | 0.98ns | 1.04ns |
Chemotype (C) | 1 | 11.38** | 1.41ns | 19.80*** | – | – | – | – |
C*G | 12 | 0.99ns | 1.01ns | 2.17* | – | – | – | – |
H2 (%) | – | 87.5 | 92.4 | 90.6 | 77.3 | 52.3 | 59.6 | 21.4 |
CDC Go | ||||||||
Gene (G) | 12 | 19.71*** | 25.19*** | 25.60*** | 11.47*** | 9.74*** | 10.92*** | 7.39*** |
Gene (entry) | 30 | 2.03** | 1.65* | 2.04** | 1.07ns | 1.03ns | 0.95ns | 1.13ns |
Chemotype (C) | 1 | 8.03* | 3.02ns | 7.38* | – | – | – | – |
C*G | 12 | 0.99ns | 1.15ns | 1.12ns | – | – | – | – |
H2 (%) | – | 80.9 | 90.4 | 86.3 | 58.0 | 56.3 | 45.0 | 15.4 |