Table 1 The primers and PCR condition for three genes
Gene | Name of primers | Sequences of primers (5′-3′) | Profiles |
|---|---|---|---|
Acc1 | Acc1F1 | CCCAATATTTATCATGAGACTTGCA | 1 cycle: 5 min 94 °C; 35 cycles: 30s 94 °C, 30s 56 °C, 2 min 30s 68 °C; 1 cycle 10 min 68 °C. |
Acc1F2 | CAACATTTGAATGAAThCTCCACG | ||
EF-G | cMWG699T3–2 | AACTGTTTTCTCATTTGTGA | 1 cycle: 5 min 94 °C; 35 cycles: 30s 94 °C, 30s 55 °C, 1 min 30s 72 °C; 1 cycle 10 min 72 °C. |
cMWG699T7–2 | AAGTGTCCTTGCCTTCCAAA | ||
ITS | ITSL | TCGTAACAAGGTTTCCGTAGGTG | 1 cycle: 5 min 94 °C; 35 cycles: 30s 94 °C, 1 min 50 s 55 °C, 1 min 50 s 72 °C; 1 cycle 10 min 72 °C. |
ITS4 | TCCTCCGCTTATTGATATGC | ||
TrnL-F | c | CGAAATCGGTAGACGCTACG | 1 cycle: 5 min 94 °C; 35 cycles: 1 min 94 °C, 1 min 55 °C, 1 min 72 °C; 1 cycle 10 min 72 °C. |
f | ATTTGAACTGGTGACACGAG |