Fig. 1

Phylogenetic tree of diploid and allopolyploid Nicotiana species. Tree reconstructed from the concatenated dataset based on maximum likelihood (ML) and Bayesian analyses is well-supported at almost all nodes. Plain nodes: ML bootstrap > 70%, posterior probability > 0.95; node with orange dot: ML bootstrap > 70%, posterior probability < 0.95; nodes with blue dot: ML bootstrap < 70%, posterior probability < 0.95; nodes with red circles: nodes of polyploid origin for estimating reconstructed progenitor phenotypes. Side flower photographs to scale, bar = 5 cm