Fig. 4
Effect of rutin on A. artemisiifolia allergen-IgE binding. a Representative slot blot membrane showing the effect of increasing concentrations of rutin on allergen-IgE binding (allergenicity). Increasing amount of rutin (ranging from 0 to 3.2 μg) were added to 2 μl of commercial pollen extract containing 3 μg of proteins, bound to nitrocellulose membrane and treated in order to assess the reaction with the pooled sera as reported in material and methods. b Quantification of immunochemical signals through image analysis: the integrated optical density (IOD) of immunoreactive spots was measured and compared with that of the standard (rutin concentration = 0). The results reported are the mean of five independent experiments. * statistically different (ANOVA, p < 0.01)