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Fig. 2 | BMC Plant Biology

Fig. 2

From: Ambrosia artemisiifolia L. temperature-responsive traits influencing the prevalence and severity of pollinosis: a study in controlled conditions

Fig. 2

Protein profile and allergen pattern of pollen from plants grown at LT and HT. a Representative 1D SDS-PAGEs stained with silver blue to show the pollen protein profiles and the related 1D immunoblot membranes probed with the same sera mix used for slot blot (allergen pattern). b Representative 2D immunoblot membranes probed with the same sera mix used for slot blot and 1D immunoblot. IgE bound proteins were identified by LC-MS-MS: (1) Amb a 12 and UDP-glucose pyrophosphorylase-like, (2) Amb a 1.03, (3) the cysteine protease Amb a 11, (4) Desiccation related protein PCC13–62-like, (5) triosephosphate isomerase like protein and Amb a 1.05, (6) Amb a 1 beta chain and Amb a 3, (7) berberine bridge enzyme-like 21, (8) glyoxal oxidase enzyme N-terminus like, (9) Amb a 1 isoforms, (10) Amb a 11, (11) triosephosphate isomerase like protein and Amb a 1.05

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