Fig. 1

Total allergenicity of pollen collected from A. artemisiifolia plants grown at different temperatures. a Representative slot blot membrane probed with a pool of selected patient sera. L: samples from plants grown at LT (18–14 °C light-dark); I: samples from plants grown at IT (24–20 °C light-dark); H: samples from plants grown at HT (30–26 °C light-dark). b: Quantification of immunochemical (IgE-binding) signals through image analysis: the integrated optical density (IOD) of immunoreactive spots with respect to the IOD of the standard was measured. The results reported are the mean of three independent experiments. Different letters indicate significant differences among the samples (Kruscal-Wallis test, p < 0.05)