Fig. 6

Candidate unigene expression levels verification and coefficient analysis of fold-change data between RT-qPCR and RNA-seq. a Transcript levels and RT-qPCR results of 7 selected genes from RNA-sequencing, which belonged to the starch and sucrose metabolism pathway. b Transcript levels and RT-qPCR results of nine selected genes from RNA-sequencing, which belonged to plant hormone metabolism pathway. The 16 unigenes were BAM9, AMY3, BAM1, SS, blgB, blgX, COI1, TPS, GID1, PYL, JAZ, PP2C, EIN3, CTR1, AHK2–3-4, and BIN2, and their full names are shown in the “Abbreviations” sections. The left y-axis shows the relative gene expression levels analyzed by qPCR (black lines). The right y-axis indicates the corresponding expression data of RNA-seq (gray histogram). The x-axis represents sampling time. The CT value of each gene was the average of three technical replicates with the standard error indicated. Significant difference of relative expression value in the different sampling times estimated by Duncan’s test was reported on the graphics (p-value < 0.05). Means labeled by the same letter are not significantly different. c Scatterplots were generated by the log2 expression ratios from RNA-seq (y-axis) and RT-qPCR (x-axis)