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Table 2 List of primary rat monoclonal antibodies used in the current study

From: Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union

Antibody

Recognized epitope

References

Hemicelluloses

 LM15

XXXG motif of xyloglucan, shows some cross-reactivity with a single galactosyl residue in xyloglucan subunits XXLG and XLXG

[81]

 LM21

β-(1 → 4)-manno-oligosaccharides in heteromannan (mannan, glucomannan, galactomannan polysaccharides)

[84]

Pectins – homogalacturonan and rhamnogalacturonan I

 LM19

unmethyl-esterified, partially methyl-esterified HG

[47]

 LM20a

methyl-esterified HG

[47]

 LM7a

partially methyl-esterified HG

[48]

 LM8

xylogalacturonan, HG domain

[85]

 LM5

linear tetrasaccharide in (1–4)-β-D-galactans (RG I side chain)

[86]

 LM6

linear pentasaccharide in (1–5)-α-L-arabinans (RG I side chain)

[87]

 LM13

longer stretches of 1,5-linked arabinosyl residues

[88]

 LM16

epitope associated with arabinans, may involve galactosyl residue(s) on RG backbones

[89]

AGPs

 JIM8b

Arabinogalactan

[90]

 JIM13

Arabinogalactan/ Arabinogalactan protein, carbohydrate epitope (β)GlcA1- > 3(α)GalA1- > 2Rha

[91]

 LM2

Arabinogalactan protein, carbohydrate epitope containing β -linked GlcA

[92]

Extensins

 LM1b

Extensin/ HRGP (epitope most likely includes extensin glycan components)

[93]

 JIM11b

Extensin/ HRGP glycoprotein

[94]

 JIM12b

Extensin/ HRGP glycoprotein

[94]

 JIM20b

Extensin/ HRGP glycoprotein

[94]

  1. aDoes not bind to unesterified HG. bEpitope structure for carbohydrate antigen: unknown. GalA galacturonic acid, GlcA glucuronic acid, Rha rhamnose