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Fig. 5 | BMC Plant Biology

Fig. 5

From: Unmethyl-esterified homogalacturonan and extensins seal Arabidopsis graft union

Fig. 5

Immunohistochemistry of grafted hypocotyl sections – homogalacturonan (LM20 and LM19 epitopes). a – weak labeling of the epidermal and cortical cell walls (arrow), no or weak fluorescence signal in the peripheral cells of the graft union (arrowheads) and endodermal cell walls (full arrow), asterisk – graft union area. a′ a, Calcofluor White. b – abundant occurrence of the epitope in the cytoplasmic compartments (arrows) and on the surface of the graft union cells located peripherally (arrowheads), asterisk – graft union area. c and d – abundant occurrence of the epitope in the cytoplasmic compartments, in the cell walls (arrows), and on the surface (arrowheads) of the graft union cells. e – epitope present in the cytoplasmic compartments (arrows), in the cell walls (full arrow), and on the surface (arrowhead) of the graft union cells. c Calcofluor White. Scale bars: a, a', and b = 50 μm; c–e = 10 μm

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