Fig. 4
From: CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean
The soybean CRISPR/Cas9-induced T4-generation spl9 mutant lines showed altered plant architecture. (a) Representative pictures showing the primary and secondary branches of wild-type (WT), GmmiR156b-overexpressing (GmmiR156b-OX) and spl9a (−/−)/spl9b-1 (−/−)/spl9c (−/−)/spl9d (−/−) quadruple mutant plants at 50th day after emergence, after the leaves were removed. Red arrows indicate the secondary branches on primary branches. (b) Node number on main stem, (c) total node number per plant, (d) branch number, and (e) dry weight were recorded after the experiment was completed at day 50th after emergence. (f) Dynamic changes in the trifoliate leaf number on main stem in different genotypes. (b-f) The data of WT, GmmiR156b-OX and the spl9b-1 (−/−) single mutant plants were obtained from six plants. The data of spl9a/spl9b-1/spl9c were obtained from five spl9a (−/−)/spl9b-1 (−/−)/spl9c (+/−) plants, the data of spl9a/spl9b-2/spl9c were obtained from nine spl9a (−/−)/spl9b-2 (−/−)/spl9c (+/−) plants, and the data of spl9a/spl9b-1/spl9c/spl9d were obtained from two spl9a (−/−)/spl9b-1 (−/−)/spl9c (−/−)/spl9d (−/−) and one spl9a (−/−)/spl9b-1 (−/−)/spl9c (+/−)/spl9d (+/−) plants. “a, b, c and d” indicate statistically significant differences among the genotypes (one-way ANOVA of variance, P < 0.05)