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Fig. 2 | BMC Plant Biology

Fig. 2

From: CRISPR/Cas9-mediated targeted mutagenesis of GmSPL9 genes alters plant architecture in soybean

Fig. 2

Schematic figure of the binary vector designed for mutagenesis of the four GmSPL9 genes using the CRISPR/Cas9 technology. The pYLCRISPR/Cas9P35S-BS was derived from the pYLCRISPR/Cas9P35S-B [38]. The target adaptor SP1, targeting two sites (GmSPL9a-SP1 and GmSPL9b-SP1), directed by the Arabidopsis thaliana U3d promoter; the target adaptor SP2, targeting two sites (GmSPL9a-SP2 and GmSPL9b-SP2), directed by the A. thaliana U6–1 promoter; the target adaptor SP3, targeting two sites (GmSPL9c-SP3 and GmSPL9d-SP3), directed by the A. thaliana U6–29 promoter; the target adaptor SP4, targeting one site (GmSPL9d-SP4), directed by the A. thaliana U3b promoter

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