Fig. 6

Effect of the compounds on actin filament and RIC4 dynamics using pollen tubes expressing Lifeact-mEGFP and CRIB4-GFP from 2 biological replicates after 2, 4 and 6 h of growth. On the right: 3D construction (pixel intensity) of RIC4 distribution in the pollen tube tip. af = actin fringe, CZ = clear zone, v = vacuole. Dashed white circle = pollen tube tip, dashed white line = pollen tube deformation, oval white circle = actin fringe accumulation, small arrow = RIC4 location at the plasma membrane and white circle = small punctate actin foci. Scale bar = 5 μm