Fig. 9From: Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traitsVegetative and reproductive development and molecular characterization of the gene tagged in the mutant Tomato ungainly leaves (Tul). a Clonal replicates of T0 plants develop leaves with a variable number of primary and secondary leaflets usually folded towards the abaxial side. b Unlike the WT (left) the basal leaves of plants cultivated in vitro of the mutant Tul (right) also exhibit a slight folding. c Adult leaves of WT (left) and mutant plants (center and right). Note the irregular and ungainly appearance of the mutant leaves. d Adult plants of WT (left) and mutant Tul (right). e Variability in the fruits of the Tul mutant. f Pollen from WT (left) and mutant plants (centre and right) stained with 0.5% TTC. Note the smaller amount of viable pollen, or even its total absence, in the anthers of mutant flowers. g Schematic representation of T-DNA insertion in the Solyc01g104410 gene. Coding sequences and 5′ and 3′ untranslated regions of the Solyc01g104410 gene are depicted as grey and striped boxes, respectively. The truncated T-DNA insertion contains the left border (LB) and the NEOMYCIN PHOSPHOTRANSFERASE II (NPTII) gene controlled by the promoter and terminator of the manopine synthase (5’mas and 3’mas, respectively. h Relative expression of Solyc01g104410 gene in wild-type and Tul mutant plants. Asterisk denotes significant differences at P < 0.05. g Spatial expression pattern of Solyc01g104410 gene in wild-type tissues. Bars (a and c) = 5 cm. Bar (b and f) =1 cm. Bar (e) = 100 μmBack to article page