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Fig. 9 | BMC Plant Biology

Fig. 9

From: Phenotypic and genetic characterization of tomato mutants provides new insights into leaf development and its relationship to agronomic traits

Fig. 9

Vegetative and reproductive development and molecular characterization of the gene tagged in the mutant Tomato ungainly leaves (Tul). a Clonal replicates of T0 plants develop leaves with a variable number of primary and secondary leaflets usually folded towards the abaxial side. b Unlike the WT (left) the basal leaves of plants cultivated in vitro of the mutant Tul (right) also exhibit a slight folding. c Adult leaves of WT (left) and mutant plants (center and right). Note the irregular and ungainly appearance of the mutant leaves. d Adult plants of WT (left) and mutant Tul (right). e Variability in the fruits of the Tul mutant. f Pollen from WT (left) and mutant plants (centre and right) stained with 0.5% TTC. Note the smaller amount of viable pollen, or even its total absence, in the anthers of mutant flowers. g Schematic representation of T-DNA insertion in the Solyc01g104410 gene. Coding sequences and 5′ and 3′ untranslated regions of the Solyc01g104410 gene are depicted as grey and striped boxes, respectively. The truncated T-DNA insertion contains the left border (LB) and the NEOMYCIN PHOSPHOTRANSFERASE II (NPTII) gene controlled by the promoter and terminator of the manopine synthase (5’mas and 3’mas, respectively. h Relative expression of Solyc01g104410 gene in wild-type and Tul mutant plants. Asterisk denotes significant differences at P < 0.05. g Spatial expression pattern of Solyc01g104410 gene in wild-type tissues. Bars (a and c) = 5 cm. Bar (b and f) =1 cm. Bar (e) = 100 μm

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