Fig. 3

Effects of expressing wild type or mutant GsCLC-c2 on salt-sensitive yeast mutant Δgef1 under salt treatments. a Survival in YPD and YPG media, and YPG media supplemented with 1 M KCl or 1 M NaCl. b Cl⁻ contents in yeast cells under 1 M NaCl treatment. S184P, E227V, E294G, C638F, A746T and I71V/C165Y are mutants with specific amino acid substituted. YPD:1% yeast extract/2% peptone/2% dextrose; YPG:1% yeast extract/2% peptone/2% galactose. Each bar represents mean ± SD of ion contents of three independent cultures of each strain. Letters indicate groups with statistically significant differences (P ≤ 0.05) using Duncan’s test after one-way ANOVA