Fig. 4
Mean N. obtusifolia PTG among normal, RNAiPIII and variant transgenic N. tabacum lines. Pollen tube length ± standard deviation was measured 40 h post pollination. The pollen tube length is the mean of five styles replicated three times. Different letters indicate a significant difference in PTG between a genotype and normal as determined by a Dunnett’s test at α = 0.05. Normal: non-transgenic N. tabacum with wildtype levels of endogenous PELPIII (S + T); Complementation lines: PIIICtrlRNAiPIII independent transformant lines 1 and 2 with the PIIICtrl and RNAiPIII transgenes; NpCtRNAiPIII independent transformant lines 1 and 2 has the NpCt (PELPIII NTD and TTS CTD) and RNAiPIII transgenes; NtCpRNAiPIII independent transformant lines 1 and 2 has the NtCp (TTS NTD and PELPIII CTD) and RNAiPIII transgenes; PIIICARNAiPIII independent transformant lines 1 and 2 has the PIIICA (cysteine to alanine mutation in PELPIII NTD) and RNAiPIII transgenes; RNAiPIII has the RNAiPIII transgene (reduced PELPIII-S and T) that was crossed with variant PELPIII transgenic lines to produce the complementation lines