Fig. 2

Gene constructs for expression of RNAi and PELPIII variants. Gene constructs used to test the hypothesis that the NTD domain is essential for PELPIII’s activity in the inhibition of interspecific PTG. All gene constructs had the sp41 modified TT-specific promoter and the 7S: 3’UTR from the 7S seed storage protein of soybean. The RNAiPIII gene construct expresses both the 3’UTR-S and the 3’UTR-T. The RNAiPIII construct has the 3’UTRsequences in the sense and antisense with a Pdk intron between them.. The Pdk intron is the pyruvate dehydrogenase kinase intron [38]. The PIIICtrl gene construct has the PELPIII NTD followed by the PELPIII CTD. The NpCt gene construct has the PELPIII NTD followed by the TTS CTD. The NtCp gene construct has the TTS NTD followed by the PELPIII CTD. The PIIICA gene construct has the PELPIII NTD with the unique cysteine mutated to alanine at position 156 followed by the PELPIII CTD