Fig. 4
From: How exposure to ALS-inhibiting gametocide tribenuron-methyl induces male sterility in rapeseed
Abnormal callose layer covering the tetrads and stopped microspore development before mitosis in TBM-treated plants. A and B are stained by aniline blue and C to F are stained by DAPI. a Callose layer covering the microsporocytes of the control. b Deformed callose layer surrounding the tetrad microspores in TBM-treated plants. c The uni-nucleate microspore of the control. d The uni-nucleate microspore of TBM-treated plants contains a weak DAPI signal. e The bi-nucleate pollen grain after first mitosis contains abundant nuclear material with a strong DAPI signal. f The nuclear materials of the TBM-treated microspore disappear at this time, and the microspores do not undergo mitosis. Abbreviations: Ca, callose; Te, tetrad. Each pair of figures had the same scale bars of 10 μm