Fig. 5

Effects of NR inhibitor (tungstate) and NOS inhibitor (L-NAME) on NO content and oxidative damage in root apices of rice seedlings. Rice seedlings were pretreated with NR inhibitor (100 μM tungstate) or NOS inhibitor (100 μM L-NAME) for 3 h, and then subjected to water treatment. (a) NO fluorescence. Bar = 300 μm. (b) NO production expressed as relative fluorescence. The contents of MDA representing lipid peroxidation (c) and carbonyl group (d) in rice seedling roots were measured after 3 h of water treatment following tungstate or L-NAME pretreatment. Values represent means± standard error (SE) (n = 6). Different letters indicate significant differences at P < 0.05. Con indicates control treatment for each N nutrition, i.e., plants receiving non-water stress. FW: fresh weight; TBARS: thiobarbituric acid reactive substances