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Table 1 Enzyme kinetic properties of three SaTPSs

From: Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum album

Enzyme

Substrate

Km (μM)

Kcat (s−1)

kcat/Km (s−1 μM− 1)

SaTPS1

GPP

9.08 ± 1.26

0.155 ± 0.007

0.0171

SaTPS2

FPP

12.15 ± 1.32

0.200 ± 0.008

0.0164

GPP

12.27 ± 1.32

0.189 ± 0.007

0.0154

SaTPS3

FPP

16.29 ± 1.48

0.171 ± 0.006

0.0105

GPP

14.38 ± 1.20

0.257 ± 0.008

0.0179

  1. Values for FPP and GPP were measured in the presence of 10 mM Mg2+
  2. All values represent mean ± SE, n = 3. Km, Michaelis constant; kcat, turnover
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BMC Plant Biology

ISSN: 1471-2229

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